Ancillaries

As a one-stop shop provider of molecular pathology workstations and reagents, we also offer a comprehensive suite of ancillary products to support our customers. These high-quality products are aimed at accelerating the generation of reproducible and reliable tissue staining results.

  1. Buffers
  2. Diluent
  3. Blocking reagents
  4. Counterstain
  5. Mounting media 
1. Buffers
Buffers and diluents are available for immunohistochemistry, in situ hybridization, special stains and most other applications.

  • General buffers, such as PBS (pH 7.6) and TBS (pH 7.6, 0.1M) can be used for washing/rinsing of slides.
  • Super Sensitive™ Wash Buffer is a phosphate buffered saline (pH 7.4) with surfactant and is used to ensure optimal staining with even spreading of antibodies and other reagents to avoid inconsistent results.
2. Diluents
  • Common Antibody Diluent and Enhanced Antibody Diluent have been developed for use with all antibodies in immunohistochemistry and have been specifically optimized for use with BioGenex antibodies and reagent products. These diluents enable enhancement of signal-to-noise ratio of staining when used optimally and also help in maintaining the antibody specificity and stability. The Enhanced Antibody Diluent, in addition to all the above features, contains chemical compounds to enhance antigen-antibody interaction and affinity. In order to achieve desired staining pattern and intensity, the titers of antibodies or concentrations of reagents may need to be optimized. These diluents are also for diluting concentrated Alkaline Phsophatase (AP) labels but are not suitable for diluting Horseradish Peroxidase (HRP) labeles because they contain Sodium Azide (0.09%).
  • Streptavidin Peroxidase Diluent, was developed especially for diluting concentrated HRP labeles and does not contain Sodium Azide.
  • Link Diluent, was developed for diluting concentrated Link (Biotinylated Anti-Immunoglobulins) antibodies
3. Blocking Reagents
  • Peroxide Block: Endogenous peroxidase is most commonly encountered in red blood cells, kidney, and liver tissue. Peroxide Block should be used prior to
    application
    of primary antibody when Horseradish Peroxidase (HRP) is used as the labeling enzyme, and if it is necessary to block endogenous peroxidase activity in the tissue being stained.
  • Power Block™: This is a blocking reagent for reducing nonspecific background in immunoassays. A truly universal block, it is suitable for use in immunohistochemistry, immunocytochemistry, ELISA methods, and immunogold techniques. The Power Block reagent contains buffer, casein
    and
    preservative and also works well as an antibody diluent and washing medium.
  • Protein Block: This can be used to reduce background staining due to
    non-specific
    binding of the primary or secondary antibodies to the tissue. Protein Block should be applied immediately prior to the primary antibody.
Product 6 mL (RTU) 50 mL (RTU)
Power Block HK083-5K HK083-50K
Protein Block / Normal Rabbit Serum (use with Goat & Rat Link) HK114-5K N/A
4. Counterstain and Mounting Media

BioGenex offers the following counterstains for use in Immunohistochemistry, in situ Hybridization and other applications with either manual or automated staining systems.

  • Mayer’s hematoxylin is a blue stain that does not contain alcohol and therefore is compatible with both alcohol soluble non-permanent chromogens (AEC, Fast Red and New Fuchsin) and alcohol-insoluble chromogens (DAB and Elegance Red). It is alcohol and xylene insoluble and therefore compatible with most clearing agents and mounting media.
Product 1 mL (RTU) 6 mL (RTU) 250 mL (RTU)
Hematoxylin, Mayer’s N/A HK100-5K HK100-9K
5. Mounting Media
Mounting of all stained biological specimens is an essential step before their microscopic evaluation. Mounting also enables the slides to be archived for long periods of time. The mounting medium may be used to attach a coverslip or may itself serve as a coverslip substitute. The choice of mounting medium depends on whether long-term or short-term preservation is desired, and whether the mounting procedure is chemically compatible with the chromogen and the counterstain.

  • SuperMount® Permanent Mounting Medium is a polymer based aqueous mounting media that does not require the use of a coverslip. This innovative, patented mounting medium (BioGenex’s U.S. Patent No. 5,492,837) is designed to preserve biological specimens for long-term storage. SuperMount® medium is compatible with most aqueous and organic-soluble dyes and chromogens including AEC, DAB, Elegance Red, Fast Red, New Fuchsin, BCIP/NBT, Rhodamine, Fluorescein, Texas Red, Phycoerythrin, Phycocyanin, and Fat Stain (Oil Red O). The refractive index of SuperMount® yields greater transparency and clarity of specimens to be examined under the microscope. SuperMount® can be used for the mounting of all biological specimens, including stained tissue sections, Cytospin preparations, and blood smears.
  • Aqueous Mounting Medium is glycerol-based mounting medium that require the use of a coverslip. It is intended for short-term specimen storage and is compatible with most chromogens and counterstains.
  • XMount™ Mounting Medium is a permanent mounting medium that has been optimized for use with BioGenex Xmatrx® instrument for all BioGenex detection systems for immunohistochemistry (IHC), In Situ Hybridization (ISH) and special stains. XMount™ is intended for use with alcohol and xylene insoluble chromogens, such as DAB (for peroxidase systems) and Elegance Red (for alkaline phosphatase systems). XMount dries clear with an ideal refractive index similar to high quality glass and tissue elements. Mounted slides can be viewed with high magnification oil immersion lenses. Also, when mounting preparations stained with the BCIP/NBT substrate, crystal formation that may occur when using other media is minimized.

Product 15 mL (RTU) Manual 50 mL  (RTU) Manual 15 mL (RTU) Xmatrx Infinity 15 mL  (RTU) Xmatrx Elite
Aquaeous Mounting Medium HK099-5K N/A N/A N/A
SuperMount® Permanent Aqueous Mounting Medium HK079-5K HK079-7K N/A N/A
Xmount™ Mounting Medium (200 tests) N/A N/A HX035-10X N/A